Abstract
Development and validation of RP-HPLC method for estimation of formoterol fumarate and budesonide in pressurised meter dose inhaler form
A reverse phase high performance liquid chromatography method was developed for the simultaneous estimation of Formoterol Fumarate and Budesonide in pressurised metered dose inhaler. The separation was achieved by octadecyl silica gel column (C18) and buffer of sodium dihydrogen phosphate and decane sulphonic acid in combination with acetonitrile as eluent, at a flow rate of 2 ml/min. Detection was carried out at 220 nm. Method was validated as per ICH guidelines and found to be stable, indicating its usefulness for analysis of said drug combination [1].Author(s): Nandini Pai and Swapnali Suhas Patil
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