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I n t e r n a t i o n a l C o n f e r e n c e o n
Neurological Disorders,
Stroke and CNS
October 22-23 , 2018
Athens , Greece
Journal of Neurology and Neuroscience
ISSN: 2171-6625
Stroke and CNS 2018
I
onic Calcium (Ca
+2
) plays an important role in controlling many physiological processes, such as muscle contractions, nerve
signaling, membrane permeability, cell division and hormone release. It is well known that in nerve cells, the excess intracellular
concentration of Ca
+2
causes cell death. In recent years, many studies have reported that certain intracellular calcium binding
proteins (CaBPs) act as calcium buffers, endogenous neuroprotectants to prevent neuronal death from excess Ca
+2
influx,
plays role in neuronal Ca
2+
signaling and Ca
+2
homeostasis, prevent or delay Ca
+2
related excitotoxicity and are involved in
neurotransmission. Besides, they are excellent markers for the neuroanatomical studies to identify unknown neuronal populations
and pathways in CNS. We have investigated the role of calb2a and calb2b genes that are expressed in the CNS and multiple
other tissues during early embryonic development of zebrafish. We have adopted individual and combined morpholino mediated
inactivation approach to investigate the functions of calb2a and calb2b. Morpholino inactivation of
calb2a/calb2b
alone failed to
generate an obvious phenotype. Morphological inspection of
calb2a/calb2b
combined knockdown morphants showed abnormal
neural plate folding in midbrain-hindbrain region. The loss of mRNA leads to severe hydrocephalus, axial curvature defect, and
yolk sac edema. Knockdown of
calb2a/calb2b
showed an impaired touchdown and swimming performance. Co-injection of the
calb2a/calb2b
morpholino oligonucleotide cocktail with human mRNA leads to the rescue of the strong morphant phenotype.
This study provided the first comprehensive analyses of the zebrafish Calb2a and Calb2b proteins that are highly conserved and
are originated from the same ancestral gene in evolution. Homology modeling and docking with the similar structure and Ca
2+
binding sites for both proteins provide the evidence that they may have similar function and one can compensate for the loss of
other. It confirms the unique and essential functions of
calb2a /calb2b
genes in the early development of the zebrafish.
agj213@hotmail.comRole of calcium binding proteins in the early
development of the zebrafish CNS
Arun G Jadhao and Rahul C Bhoyar
RTM Nagpur University, India
J Neurol Neurosci 2018, Volume: 9
DOI: 10.21767/2171-6625-C3-015