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I n t e r n a t i o n a l C o n f e r e n c e o n
Neurological Disorders,
Stroke and CNS
October 22-23 , 2018
Athens , Greece
Journal of Neurology and Neuroscience
ISSN: 2171-6625
Stroke and CNS 2018
T
his experimental study assessed the effects of riboflavin on motor disability, spatial learning, and memory in experimental
autoimmune encephalomyelitis (EAE). The C57BL/6 female mice (n=56) were assigned into 7 groups: sham operated 1 (SO1),
healthy mice received PBS (phosphate buffer saline); sham operated 2 (SO2), healthy mice received PBS and riboflavin; sham
treatment1 (ST1), EAE mice received water; sham treatment 2 (ST2), EAE mice received sodium acetate buffer; treatment 1
(T1), EAE mice received interferon beta-1a (INF-β1a); treatment 2 (T2), EAE mice received riboflavin; treatment 3 (T3), EAE mice
received INFβ-1a and riboflavin. After EAE induction, scoring was performed based on clinical signs. By detecting score 0.5,
riboflavin at 10 mg/kg of body weight and/or INFβ-1a at 150 IU/g of body weight, administration were started for two weeks. The
brain and spinal cord levels of brain-derived neurotrophic factor (BDNF) were studied using real-time PCR and ELISA methods.
Spatial learning and memory were assessed through the Morris water maze (MWM). BDNF mRNA expression and BDNF levels
increased significantly in the brain of T3 group compared to the T2 or T1 groups (
P<0.01
and
P<0.05
, respectively). Clinical
scores were reduced in riboflavin treated groups compared to others. The EAE mice performed similarly compared to the healthy
controlled mice in MWM test. However, T2 and T3 mice swam faster than the ST2 (P<0.05), T1 (
P<0.05
), and ST1 (
P<0.05
) mice.
The results concluded that riboflavin has beneficial effects on neurological motor disability mediated by BDNF in EAE model of
MS.
mnaghashpour@gmail.comRiboflavin: beneficial effects on neurological
motor disability but not spatial learning and
memory consolidation in murine model of multiple
sclerosis
Reza Amani
1
and Mahshid Naghashpour
2,3
1
Food Security Research Center-Isfahan University of Medical Sciences, Iran
2
Abadan School of Medical Sciences, Abadan, Iran
3
Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sci-
ences, Iran
J Neurol Neurosci 2018, Volume: 9
DOI: 10.21767/2171-6625-C3-015