First-derivative spectrophotometry, applying the peak-zero method, was developed for the determination of Gliclazide in tablets. The solutions of standard and sample were prepared in 0.1N NaOH. Quantitative determination of the drug was performed at 226 nm,217nm for zero order and first order respectively and was evaluated for the parameters specificity, linearity, precision, and accuracy. The specificity test showed that there was no interference from excipients commonly found in the commercial pharmaceutical formulation at 226 nm and 217nm. The standard curve showed a correlation coefficient of 0.99992 for zero order spectroscopy and 0.99999 for first derivative method. Precision was demonstrated by a standard deviation value of 0.838.The recovery test resulted in an average of 100.48%, and 100.64% for zero order and first order respectively which confirmed the accuracy of the method.
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