U.V. Spectroscopy technique for analysis of amoxcillin trihydrate in pH stimuli sensitive formulation

The present study describes a simple, specific, sensitive, rapid and economical u.v. spectroscopy method for estimation amoxicillin trihydrate concentration in pH sensitive formulation. The absorption maxima or λmax of the drug was found to be 334.5 nm. A linear response was observed in the range of 2-50 μg/ml in simulated buffer solution (pH 1.2) and phthalate buffer solution (pH 3.4) with a regression coefficient (r2) of 0.9812 and 0.9808 respectively. The method was then validated like slope, intercept , standard deviation , limit of detection (LOD), and the limit of quantification (LOQ) were found to be 0.075 , 0.069 , 0.042, 1.68 μg/ml , 5.6 μg/ml respectively whereas in phthalate buffer (pH 3.4) and value of slope, intercept, correlation coefficient, standard deviation , LOD and LOQ were found to be 0.084 , 0.032 , 0.046 , 1.642 μg/ml, 5.47 μg/ml respectively. The low values of LOQ and LOD of the proposed method can be used for the determination of amoxicillin trihydrate in quality control of acidic pH sensitive formulation without interference of the excipients.

Author(s): Girish Kumar Tripathi

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