Stability indicating HPLC method for simultaneous determination of methocarbamol and nimesulide from tablet matrix

A reverse phase HPLC method has been developed for simultaneous estimation of methocarbamol and nimesulide in a tablet formulation. The separation was achieved by using Supelcosil LC-8 (4.6 mm I.D x 250 mm) column and methanol, water (60, 40 v/v) as an eluent, at flow rate of 1.0 mL/min. Detection was monitored at 276 nm. The retention time of methocarbamol and nimesulide was found to be 3.81 and 5.93 min respectively. The validation of the proposed method was also carried out for linearity, accuracy and precision. The dynamic range for methocarbamol and nimesulide was 0 to 0.5 mg/mL and 0 to 0.05 mg/mL respectively. The percentage recoveries obtained for methocarbamol and nimesulide were 100.39 and 99.74 respectively. The developed method was found to be simple, precise and accurate, and it can be used for routine quality control analysis of these drugs in combination tablets.

Author(s): R. S. Manmode1, A. K. Dhamankar2, J. V. Manwar3* and S. S. Laddha4

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