Spectrophotometric methods for the determination of emtricitabine in bulk and in its pharmaceutical formulations using aromatic aldehydes as chromogenic reagents

Two simple, accurate, sensitive and extraction-free spectrophotometric methods (A and B) have been developed for the determination of emtricitabine in bulk and in its pharmaceutical dosage forms. Both A and B methods are based on the reaction of emtricitabine with p-Dimethylaminobenzaldehyde (PDAB) and 4-Hydroxy-3-methoxy benzaldehyde (HMBA) in the presence of dilute sulphuric acid gives red colored chromogens, which show maximum absorbance at 495nm and 480nm respectively. Beer’s law is obeyed in the concentration range 1.0-40.0 μg mL-1 for method A, and 2.5-35.0μg mL-1 for method B respectively. The molar absorptivity and sandell’s sensitivity values are 6.1 x103 L mo1e-1cm-1 and 0.04 μg cm-2for method A, and 6.9 x103 L mo1e-1cm-1and 0.035 μg cm-2 for method B respectively. The results of these methods are validated statistically and by recovery studies. The proposed methods are economical, sensitive and can be used for the determination of emtricitabine in bulk and in its pharmaceutical formulations.

Author(s): P. Saifulla Khan

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