Molecular variations were investigated in four rabbit lines namely Alexandria, V-line, New Zealand white and California in order to find special molecular genetic markers which can differentiate among studied lines. Five out of 10 RAPD primers exhibited sufficient variability and were used to characterize the genetic similarity and diversity among studied lines. A total of 198 DNA bands were detected. Phylogenetic analysis using Nei and Hi method generally produced four clusters which were completely distinguished based on the locality of the rabbits. From the phylogenetic tree, the genotypes Alexandria and V-line appeared to be closely related, and also the genotypes V-line and California. So, the results indicated the efficacy of RAPDPCR technique to detect similarity among rabbit genotypes and their applicability in establishing genetic relationships among them.
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