Abstract

Molecular Therapeutic Role Micro-RNA 221 Inhibitor in Hepatocellular Carcinoma

Background: MicroRNAs (miRNAs) have plays important role not only as posttranscriptional regulation of gene expression also in development and cellular processes. The association between pathogenesis of many diseases and miRNAs very strong, between pathogenesis many diseases and miRNAs very strong, miR-221 is an oncogenic miRNA that plays important role in the initiation and progression of HCC, by affecting pro-oncogenic pathways from that miRNAs is a new class of targets for the development of miRNA-based therapeutic strategy.

Method: The mature miRNA act by competition with cellular target miRNA act by completion with cellular target mRNAs moreover that lead to inhibition of the miRNA and repression of the its direct targets. For assessment effect of anti-miR-221 on HepG2 cells, MTT, cell cycle analysis and apoptosis assay were performed, as regard expression -miR-221 and its target gene were detected by using reverse transcription-polymerase chain reaction.

Results: Our data revealed that the delivery of anti-miR-221 that mediated by ultrasound micro bubble contrast agents. We observed that, cell proliferation inhibited and promoted the apoptosis of cells the expression of miR-221 decreased. Consequently, there was up regulation in the expression of CDKN1B/p27.

Conclusion: The transfection of recombinant plasmids contained anti-miR-221 to suppress oncogenic effect of-miR-221 and cell proliferation was inhibited and cell apoptosis was induced in HepG2 cells. This indicated that effective role miRNA that may be novel gene therapy targets.


Author(s): Amal Abou Elfadl, Naglaa I Azzab, Sania K Elwia, Doaa Shabaan

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