Kinetic model for the quantitative determination of starch in large number of biological samples

The starch quantification was achieved through the controlled kinetic model of acid hydrolysis of potato starch at pH 3.0. Potato starch (weight average granule diameter, 40.0 μm) was hydrolyzed in a batch reactor using 0.001M HCl (pH 3.0) solution. The conversion of starch into reducing sugar glucose was recorded separately, at different temperatures (343K, 353K and 363K) as a function of time. Maximum (37.92%) conversion of starch to glucose (in 90 minutes) was obtained at 363K. Reaction follows first-order rate equation with respect to starch concentration. Based on the rate constant (k) and half-life (t1/2) of acid hydrolysis, unknown samples were quantified for their starch content as per the derived kinetic model. The method was found suitable for quantification of large number of biological samples of similar type in lesser time.

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