A study was conducted at Metahara Sugar Estate of Ethiopia Sugar Industry with the objective to determine the effects of different concentrations of IBA and immersion time on ex vitro root induction of detached sugarcane plantlets. Five levels of IBA (0, 0.5, 1, 1.5 and 2 mgL-1) and four levels of immersion time (0, 1, 5 and 10 minutes) with three sugarcane genotypes were combined in factorial treatment combination arrangements with completely randomized design in pot experiment under nursery. The basal end of the detached shoots was dipped in IBA solution for different durations before the shoots were transferred into a polyethylene bag containing a mixed growing medium. The results showed that the interaction effect of IBA, immersion time and the sugarcane genotypes was very highly significant (p<0.0001) on number of roots per shoot, average root length and average survival rate of the sugarcane plantlets. Sugarcane plantlets of SP7-1284, C132-81 and C86-56 dipped in 0.5 mgL-1 IBA for 10 minutes gave the optimum rooting responses with highest survival rate in all the sugarcane genotypes studied.