Bacterial drug resistance is one of the major factors which plays a crucial role in the treatment. Bacteria evolved various mechanisms to evade the action of these antibiotics. One of the strategies is the prevention of binding of antibiotics to ribosome through addition of methyl group favored by different methyltransferase enzymes. Ribosomal methylation leads to development of multi-drug resistant organisms, which are resistant to different classes of antimicrobial agents. Development of antibiotic resistance due to the ribosomal methylation raise concerns about the future clinical efficacy of several antimicrobial classes.