Development of analytical method for pyrantelembonate using spectrofluorometry

A simple, robust, selective and sensitive spectrofluorometric method has been developed for the determination of pyrantel embonate in bulk and pharmaceutical formulations. The method was based on the scanning of aqueous solution of the drug and formulation. The fluorescence was measured at the excitation of 235 nm and the emission was determined at 520 nm. The method showed high sensitivity with linearity range from 4 to 24 µg/ml. The lower limit of detection (LOD) was found to be 0.66 µg/ml and the limit of quantization (LOQ) was determined as the lowest concentration was found to be 2.2 µg/ml. The variables that affected the reaction were carefully studied and optimized. The proposed method was applied successfully for the determination of pyrantel embonate in bulk and pharmaceutical formulations. The mean percentage recovery is was found to be 101.16±1.057.

Author(s): K. Gnanaprakasam and A. Anton Smith

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