Development and validation of liquid chromatographic method for atenolol and its related substance

A simple isocratic, rapid and sensitive high performance liquid chromatographic method has been developed for quantitative determination of Atenolol and its three process related impurities. The method has been validated for determination of related substance in Atenolol. Analytical Column: A stainless steel column 150 mm long, 4.6 mm internal diameter filled with octadecylsilane chemically bonded to porous silica particles of 5 μm diameters (Use Chromatopak Peerless basic C 18, 5 μ, 150 mm x 4.6 mm). Pump mode : Isocratic wavelength: 226 nm, Flow rate : 1mL per minute, Injection volume : 50 μl, Run time: 30 min. Specificity, system suitability, linearity, precision, ruggedness, robustness along with limit of quantification and limit of detection have been carried out for Atenolol and its process impurities.
Author(s): Nandini R. Pai and Swapnali Suhas Patil

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