Development and Validation of HPTLC Method for Simultaneous Determination of Wedelolactone and Phyllanthin in Hepasuport Tablet

A new and simple HPTLC method was developed and validated for the simultaneous quantitative determination of Wedelolactone and Phyllanthin in hepatoprotective Hepasuport tablet. TLC aluminium plates precoated with silica gel 60F-254 (0.2 mm thickness) were used. The samples were dissolve in methanol and linear ascending development was carried out in twin trough glass chamber saturated with mobile phase Toluene: Ethyl acetate: Formic acid (5.0:4.0:1.0, v/v/v) and densitometric determination of these compounds was carried out by TLC scanner (CAMAG) at 254 nm in reflectance/absorbance mode. The Rf value of wedelolactone and phyllanthin was found to be 0.56 ± 0.02 and 0.65 ± 0.03 respectively. The method was validated with respect to specificity, linearity, accuracy, precision, ruggedness and robustness as per the International Conference on Harmonization (ICH) guidelines. Results found to be linear in the concentration range of 600ng to 1600 ng with r2 = 0.998 and 0.995 for wedelolactone and phyllanthin, respectively. The percent recoveries were found to be 100.06 and 99.35 for wedelolactone and phyllanthin respectively. The method can be used for routine analysis of these phytoconstituents in tablet dosage forms in quality control laboratories.

Author(s): Milind N. Prajapati Tushar A. Deshmukh, Vijay R. Patil, B. K. Shrikhande

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