Cytotoxic and Radical Scavenging Potential of Indian Almond (Terminalia catappa) Leaf Extracts

Solvent leaf extracts of Terminalia catappa were evaluated for their cytotoxic and their radical scavenging potential. Brine shrimp assay was used for exploring cytotoxic activity, all the extracts namely hexane, DCM, chloroform, acetone and methanol were tested at the dose level of 25, 50, 100 and 200microgram /ml. A dose dependent response was obtained in all the extracts. Chloroform extract showed 100percent activity at the highest dose of 200µg/ml. Antioxidant activity was evaluated by one qualitative(TLC based) and two quantitative radical scavenging assays namely DPPH (2, 2- Diphenyl-1- picryl hydrazyl) assay and ferric reducing antioxidant power (FRAP) assay. In both assays acetone extract exhibited significant reduction of free radicals. The IC50 value of acetone extract was 36.9µg/ml. Ascorbic acid was used in these assays as reference compound. FRAP value of acetone extract was found to be 6.73 much higher than all the other extracts.

Author(s): D. R. Behera, Sunita Bhatnagar and A.K.Mahapatra

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