Epstein-Barr virus (EBV) considers the first human virus to be directly implicated in carcinogenesis in different sites of body. Prostate adenocarcinoma is very common in all world. In Iraq, prostate cancer is one of the ten top cancers that affect men. 30 formalin-fixed Paraffin embedded tissue blocks were obtained from prostate carcinoma biopsies and transurethral Resection of Prostate (TURP) and, 30 formalin-fixed, paraffin embedded blocks were obtained from benign prostatic hyperplasia (BPH) collected from archives of laboratory in Ghazi Al-Hariri hospital for surgical specialties and from private histopathology laboratories in Baghdad from May 2016 to December 2016, and 20 control tissue from forensic medicine institute in Baghdad. All samples examination by In situ hybridization (ISH) to detect Epstein-Barr encoded small RNAs (EBERs), then use Immunohistochemistry (IHC) to exam EB nuclear antigen-2(EBNA-2) and Latent membrane protein-1(LMP-1). EBV can be detected by successful method In situ hybridization technique' to exam EBERs in Prostate cancer, which detected only in Prostate carcinoma groups in this study. LMP-1 and EBNA-2, can be detected by Immunohistochemistry method in Prostate carcinoma with highly percentage than benign prostate hyperplasia and control group. Finally, there is a correlation between EBERs, LMP-1 and EBNA-2 in malignant group.