Analysis of methotrexate in pharmaceutical formulations by UV-Visible spectrophotometric method

A simple, sensitive, selective rapid spectrophotometric method has been developed for the determination of post synaptic α1- Adrino receptor antagonist Methotrexate in pure form and pharmaceutical formulations based on the oxidative coupling reaction with 2, 2- Bipyridine reagent, at PH_4.0 which is extractable at 510 nm. Beer’s law is obeyed in the concentration ranges 4-24 μg ml-1. The developed method was applied directly and easily for the analysis of the Pharmaceutical formulations. R.S.D was found to be 0.2859% and Recovery 98.99% respectively. The method was completely validated and proven to be rugged. The interferences of the other ingredients and excipients were not observed. The repeatability and the performance of the proved method were established by point and internal hypothesis and through recovery studies.
Author(s): Bandi Ramachandra, P. Suguna and N. V. S. Naidu

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