Glimepiride is a long acting Sulfonylurea imitative has its place to third generation sulfonylurea. It finds its application in the management of non-insulin contingent Type 2 diabetes mellitus and it is having a medium to long acting antidiabetic activity. For the persistence of clinical and pharmaceutical analysis, fruitful analytical procedures are obligatory to study quality control, pharmacodynamics and pharmacokinetic properties and stability studies as well. There are plenty of articles which have already been published describing analytical methods and method validation for the same. In present review account, the disclosed analytical methods are outlined for the establishment of Glimepiride in its pharmaceutical preparations and biological matrices. Most frequently used techniques such as spectrometric and liquid chromatographic methods are summarized in present review. Spectrometric methods for Glimepiride alone and in combination include parameters like λ max, solvent, matrix etc. and HPLC methods for Glimepiride alone and in combination including parameters like matrix, stationary phase, mobile phase composition detection wavelength etc. HPTLC methods including parameters like stationary phase, mobile phase combination, RF etc.