Next Generation Sequencing (NGS) is a ground-breaking stage that has empowered the sequencing of thousands to a great many DNA particles at the same time. Next Generation sequencing (NGS), otherwise called high-throughput sequencing, is the trick all term used to depict various distinctive current sequencing advancements. The appeal for ease sequencing has driven the improvement of high-throughput sequencing which produce thousands or a huge number of successions without a moment's delay. They are planned to bring down the expense of DNA sequencing past what is conceivable with standard color eliminator techniques. Along these lines, these ongoing advances permit us to arrangement DNA and RNA significantly more rapidly and inexpensively than the recently utilized Sanger sequencing, and as such have altered the investigation of genomics and atomic science Ordered to various ages, NGS has prompted beaten the confinements of traditional DNA sequencing techniques and has discovered use in a wide scope of sub-atomic science applications