Lc Ms Applications

The LC-MS technique is useful for the detection of steroid drugs in body fluids and in profiling endogenous steroids. Steroid sulfates have been detected with high sensitivity using this method. Plasma spray has been used to test saliva for steroid hormones in patients suffering from congenital adrenal hyperplasia. Amino acids were one of the first compounds analyzed using LC-MS coupled with laser desorption and thermospray. Nucleosides, nucleotides, saccharides, peptides, and proteins were all analyzed and their molecular weights were determined using LC-MS coupled with electrospray. Bile acids have also been determined using LC-MS and thermospray. Ultra-pure additives and solvents such as acetic acid, acetonitrile, ammonium acetate, ammonium bicarbonate, ammonium fluoride, and ammonium hydroxide are available from Sigma Aldrich for use in LC-MS systems. LC-MS coupled with laser desorption and thermospray are beeter even at bioinformatics part to uderstand easily.

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