Bioinformatics was coined by Paulien Hogeweg and Ben Hesper in 1970. It was stated as "Study of Informatic processes in biotic systems". Basically bioinformatics deals with the information in the fields of Information Technology, Computer Science and Biology. Biologist performs research in laboratoty and collects DNA and protein sequences, gene expressions etc. Computer Scientists are involved in developing algorithms, tools, softwares to store and analyze data. Bioinformaticians study biological questions by analyzing molecular data with various programs and tools. Today, bioinformatics is used in large number of fields such as microbial genome applications, biotechnology, waste cleanup, Gene Therepy etc. In this article an effort is made to provide brief information of applications of bioinformatics in the field of Medicine, Microbial Genome Application and Agriculture.