Archives of Clinical Microbiology

contact@imedpub.com Submit a Manuscript

Inoculum Development

The development of active logarithmic microbial culture that is suitable for the final industrial production level is known as inoculum development. Inoculum we use for industrial fermentations should be • In its active, healthy, and exponential growth phase. • Available free of contamination required large volumes. • Retain its capability of the formation of desired product formation. The process is a stepwise and gradual increase in scaling-up the volume of inoculum to the desired level, which includes preparation of bacterial suspension (either vegetative cells or spores) in sterile tap water and then to the broth or in case of fungi, their hyphae are transferred to the broth.  A two-stage inoculum development program is used for the production of proteases by Bacillus subtilis. The process is a stepwise and gradual increase in scaling-up the volume of inoculum to the desired level, which includes preparation of bacterial suspension (either vegetative cells or spores) in sterile tap water and then to the broth or in case of fungi, their hyphae are transferred to the broth. This is usually done using flask cultures ranging in between 50 ml to 12 lt and the volume of the flask or container can be increased as per the need. The volume of inoculum we add to fermenter should be about 5 % to that of media volume. Major pitfalls we should be aware of are the risk of contamination and the degeneration of strain. Peer review refers to the work done during the screening of submitted manuscripts and funding applications. This process encourages authors to meet the accepted standards of their discipline and reduces the dissemination of irrelevant findings, unwarranted claims, unacceptable interpretations, and personal views. Publications that have not undergone peer review are likely to be regarded with suspicion by academic scholars and professionals.

High Impact List of Articles
Conference Proceedings

Relevant Topics in Immunology & Microbiology

Awards Nomination 17+ Million Readerbase
Google Scholar citation report
Citations : 1379

Archives of Clinical Microbiology received 1379 citations as per Google Scholar report

image
Abstracted/Indexed in
  • Google Scholar
  • Open J Gate
  • Genamics JournalSeek
  • The Global Impact Factor (GIF)
  • Open Archive Initiative
  • China National Knowledge Infrastructure (CNKI)
  • Scimago
  • Directory of Research Journal Indexing (DRJI)
  • WorldCat
  • Proquest Summons
  • Publons
  • MIAR
  • ResearchGate
  • University Grants Commission
  • Geneva Foundation for Medical Education and Research
  • Secret Search Engine Labs

View More »

Open Access Journals