Biofilm is an association of microorganisms in which they are adhere to each other on a living or non-living substances within an extracellular polymeric substance, Microorganisms that form biofilms include bacteria, fungi and protists, when free floating microbes come in contact with an appropriate substance and produce a gooey substance known as extracellular polymeric substance(EPS), EPS is a network of proteins, nucleic acids, sugars.

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