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conferenceseries
.com
Volume 8, Issue 6
J Neurol Neurosci
ISSN: 2171-6625 Neuro, an open access journal
Neuroscience 2017
October 16-17, 2017
OCTOBER 16-17, 2017 OSAKA, JAPAN
17
TH
Global Neuroscience Conference
CNS lymphatic drainage blockade exacerbates cerebral vasospasm and cerebral injury following
subarachnoid hemorrhage and partially reversed by
Ginkgo biloba
extract
Arunkumar Prasad, Bao-Liang Sun, Xuan Wang, Li Jia, Li-Li Jia, Xi-Chang Liu, Zi-Cui Cheng, Ming-Feng Yang, Cheng-Bi Zhengb, Lei-Lei Mao, Cun-Dong
Fan, Zong-Yong Zhang, Da-Wei Li, Xiao-Yi and Yang
Taishan Medical University, China
R
ecent studies suggest that CNS lymphatic drainage pathway to extra-cranial lymph compartments may play an important
role in the removal of substances in the brain and Cerebrospinal Fluid (CSF). After the onset of Subarachnoid Hemorrhage
(SAH), large amount of macromolecular substances, such as cellular lysates, proteins, peptides, were accumulated in the
brain tissue and CSF, which contribute to cerebral vasospasm and cerebral injury. The present experiment was carried out
to investigate the possible role of cerebral lymphatic drainage pathway in the development of cerebral vasospasm and related
cerebral injury and the influence of Ginkgo biloba extract. Wistar rats were used in the experiment and animals were divided
into different groups. SAH models were replicated by double cisternal injection of autologous arterial hemolysate. In some
animals the main cerebral lymphatic drainage way out being blocked (Cerebral Lymphatic Blockade, CLB). Two different
constituents, ginkgolides and ginkgo flavone, were given as interventions. It was found that SAH reduced the drainage of
Evans Blue-Labeled Albumin (EBA) from the brain to the olfactory bulbs, cervical lymph nodes and abdominal para-aortic
lymph nodes. A kinetic analysis of 125I-labeled human serum albumin (125I-HSA), a Cerebrospinal Fluid (CSF) tracer,
showed that the clearance rate of macromolecules in the CSF was significantly reduced after SAH. Furthermore, SAH reduced
the diameters of Basilar Artery (BA) and increased thickness of BA. Prominent cerebral injury was found after induction of
SAH. The spasm of BA and cerebral injury were partially antagonized by ginkgolides and ginkgo flavone. It was concluded
that cerebral lymphatic drainage pathway exerts intrinsic protective effects against cerebral vasospasm and cerebral injury by
removal of macromolecular substances in the brain and subarachnoid spaces. Ginkgolides and ginkgo flavone may alleviate
the exacerbated cerebral vasospasm and cerebral injury following SAH by CLB.
Biography
Arunkumar Prasad is the President of the student organization in the field of academics and other activities in TSMU. He is currently working as a Young Researcher
and pursuing his studies under guidance of Professor Baoliang Sun.
arunp832@gmail.comArunkumar Prasad et al., J Neurol Neurosci 2017, 8:6
DOI: 10.21767/2171-6625-C1-005