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conferenceseries
.com
Volume 8, Issue 6
J Neurol Neurosci
ISSN: 2171-6625 Neuro, an open access journal
Neuroscience 2017
October 16-17, 2017
OCTOBER 16-17, 2017 OSAKA, JAPAN
17
TH
Global Neuroscience Conference
Prenatal and early postnatal caffeine exposures influenced neuron and glial morphological destinies
Owolabi O Joshua
1
, Adefule A K
2
and Shallie Philemon D
2
1
Babcock University, Nigeria
2
Olabisi Onabanjo University, Nigeria
T
his study investigated the effects of caffeine consumption on the brain in pregnancy and early postnatal life. Thirty two
(n=32) adult mice (
Mus musculus
) were used for the study. They were maintained in Anatomy Department animal holding
and given chow and water ad libitum. They were divided into four groups: Group-A animals as control, Group-B animals given
low dose caffeine 10 mg/kg body weight, Group-C animals given medium dose caffeine 50 mg/kg body weight and Group-D
animals given high dose caffeine 120 mg/kg body weight. The treatment duration was divided into Phases I and II to assess
pre and post natal effects, respectively. Caffeine was dissolved in distilled water and Group-A animals received distilled water
as placebo. Animals mated prior to treatment commencement and pregnant mice were administered caffeine throughout
pregnancy. Half number of offspring were sacrificed at birth, the rest were used in Phase II and treatment continued till postnatal
Day 35, marking puberty. Animals were sacrificed and brain specimens were excised and processed. Specimens were subjected
to gross morphological assessments. Histological and histochemical demonstrations were done using the H&E, Bielschowsky,
Luxol Fast Blue and Feulgen DNA Staining techniques. Immuno histochemical-molecular properties were demonstrated using
the GFAP technique. At parturition, 50 mg/kg body weight caffeine dose disrupted deeper cortical layers histoarchitecture
and extended to other layers when dosage increased to 120 mg/kg body weight. Neuronal morphological heterogeneity was
observed at doses higher than 10 mg/kg body weight and some effects persisted till puberty. Astrocyte morphologies and
dendritic patterns of elaboration were persistently altered. Caffeine exposure altered the pattern of brain cells development by
altering morphologies, patterns of elaboration and spatial distribution, also neuronal communication and basal metabolism.
Effects were dose dependent and moderate dose might be beneficial to morphological and functional parameters.
Biography
Owolabi O Joshua has received his PhD in Anatomy and Neuroscience and MBA with specialization in Clinical Research Management. He currently works as a
Teacher and Researcher in the Ben Carson [Snr.] College of Medicine, Babcock University, Nigeria.
owolabijo@babcock.edu.ngOwolabi O Joshua et al., J Neurol Neurosci 2017, 8:6
DOI: 10.21767/2171-6625-C1-005