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Microbiology & Virology 2018
J u n e 2 1 - 2 2 , 2 0 1 8
P a r i s , F r a n c e
Page 36
Archives of Clinical Microbiology
ISSN 1989-8436
E u r o S c i C o n C o n f e r e n c e o n
Microbiology &
Virology
Soil environment is polluted with heavy metals as a result of increasing
industrialization, mining and more advancement in technology. Some
of the main contaminations are controlled and uncontrolled discharge
of solid and liquid wastes, accidental spillages, herbicides, insecticides,
pesticides, agriculture fertilizers, sewage disposal, explosives and tar into the
environment. Worldwide major health alarming related to random release of
heavy metals into the soil and waters and exposure of extreme concentrations
of these heavy metals can cause rigorous harmful effects to animals, plants
and human, as they cannot be converted to non-toxic forms and therefore have
long-term effects on the flora and fauna. The current study was focused on
isolation, phenotypical and genotypical characterization of metallotolerant
indigenous fungal strains by using heavy metal contaminated soil of Hattar
Industrial Estate, Haripur. First, these isolates were identified based on their
morphological and microscopic analysis. Then, they were confirmed by
amplification and analysis of their specific internal transcribed spacers (ITS)
sequences, allowed discrimination of the metallotolerant indigenous fungal
isolates as Aspergillus nigerM1, Aspergillus flavusM2, Penicilliumchrysogenum
M3, Aspergillus parasiticus M4, Pencillium notatum M5, Aspergillus terreus
M6, Fusarium solani M7 and Aspergillus fumigatus M8. Furthermore, all the
isolated fungal strains were found highly resistant to heavy metals especially
to Cadmium (cd), Copper (cu), Lead (Pb) and Mercury (Hg). Thus heavy
metal contaminated soil might be considered as a precious natural source of
resistant fungal strains, which can be used significantly as a bioleaching or
bioremediation tool.
Biography
Ibrar Khan has completed his PhD fromOcean University of Chi-
na. He is an Assistant Professor at Department of Microbiology,
Abbottabad University of Science & Technology, Pakistan. He
has published more than 20 papers in reputed journals.
Isolation, phenotypic and genotypic characterization of
metallotolerant fungal isolates from industrial soil
Ibrar Khan1,2 , Maryum Aftab2, Isfahan Tauseef2 and Kashif Syed
Haleem2
1Abbottabad University of Science & Technology, Pakistan
2Hazara University, Pakistan
Ibrar Khan et al., Arch Clin Microbiol 2018 Voulume: 9
DOI: 10.4172/1989-8436-C3-011