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Microbiology & Virology 2018
J u n e 2 1 - 2 2 , 2 0 1 8
P a r i s , F r a n c e
Page 32
Archives of Clinical Microbiology
ISSN 1989-8436
E u r o S c i C o n C o n f e r e n c e o n
Microbiology &
Virology
T
he emerging future of antisense oligonucleotides depends on rational
modifications of its nucleotide repeating units. Currently themost prevalent
design features that are crucial for continued antisense development are
nuclease resistance, cellular uptake, hybridization properties, and disruption
of RNA functions through terminating events.Very few structure-activity
relationship (SAR) studies have been directed to these problems and these
have typically used binding affinities and nuclease sensitivities as activity
end points rather than in vitro or in vivo biological activities. Further SAR
studies may be approached by sequence selection SARs which hold a certain
uniform modification type constant (e.g. phosphorothioates) while varying
the sequence of the oligonucleotide.The more traditional approach is to
modify the oligonucleotide while keeping the sequence constant. This review
is concerned with the latter approach and summarizes modifications of
the phosphorus atom, pentofuranosyl (sugar) linker, pentofuranosyl ring and
its 2'-substituents, and the heterocycles. The review covers the 1989-91
literature of various modified oligonucleotides designed and synthesized
to enhance pharmacokinetic and pharmacodynamic properties of antisense
oligonucleotides.
Keywords-
oligonucleotides, nuclease, RNA, SAR, pentofuranosyl, hetercycles.
Biography
Mohammed Sissaoui has completed his Master's degree In
Biochemistry & Molecular Biology at the age of 30 years from
The University Of Biskra - Algeria. He is a Medical Laborotry Sci-
entist At the Hospital Of Taoura Souk Ahras - Algeria, and
has been serving as a Medical Laboratory Technologist at
The Hemodialysis Clinic Souk Ahras - Algeria.
sissaoui_mohammed@yahoo.comAnti-cancer Drug Design
M.SISSAOUI
University of Biskra, Algeria
M.SISSAOUI, Arch Clin Microbiol 2018 Voulume: 9
DOI: 10.4172/1989-8436-C3-011