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Microbiology & Virology 2018
J u n e 2 1 - 2 2 , 2 0 1 8
P a r i s , F r a n c e
Page 35
Archives of Clinical Microbiology
ISSN 1989-8436
E u r o S c i C o n C o n f e r e n c e o n
Microbiology &
Virology
Staphylococcus aureus is an opportunistic human and animals’ pathogen
that is found passively colonizing a large percentage of the population
primarily on the skin or mammary glands in cows. As the capacity of a given S.
aureus strain to cause infection varies among strains and is determined by the
presence or absence of a large number of virulence-associated genes, some
of which are encoded by phages. In order to determine the abundance and
characterize the diversity of S. aureus strains which caused mastitis in cows in
various provinces in Baghdad city, polymerase chain reaction (PCR) was used
to detect the presence of S. aureus phage-type specific DNA which carry genes
coding for diverse virulence factors such as Panton-Valentine leukocidin
(PVL), enterotoxins and exfoliative toxins. Staphylococcus aureus used in this
study was isolated from mastitic cow milk grew on blood agar and methicillin-
resistant Staphylococcus aureus (MRSA) were selected as indicator strains to
investigate the presence of phage segments. The isolates of wild lytic phages
from the transient stocks were propagated with the corresponding host clinical
MRSA isolates using the plate method. Plates were incubated overnight at 37˚C
and plaque morphology and growth characteristics were recorded. Genomic
DNA from isolated S. aureus phage was extracted and phage segments were
investigated. The results showed that the phage content virulence factors in S.
aureus isolates were enterotoxin A, enterotoxin P, and exfoliative toxin A, toxic
shock syndrome toxin-1, lipase. But Panton-Valentine leukocidin gene failed to
amplify among strains and the diversity of it in Baghdad city is relatively high.
The current study concluded that there is role of bacteriophages in changing
ecology and virulence of S.aureus .
Biography
Atheer is microbiologist work in university of Baghdad, veteri-
nary medical college , department of microbiology since nine
years. I got my . in science college university of Baghdad
department of biology in 1984 and work in al Mansur factory as
a microbiologist for water treatment, then I got MSc in bacteri-
ology in veterinary college and work head department of bacte-
riology in Al razee center for research and production diagnostic
kits in ministry of industry and minerals, after that I got my PhD
in virology from university of Almustanseria department of biol-
ogy and return to my work, head department of virology ,from
this time I started to work in research and published my work in
many journals. Many research were publish in rotavirus ,cholera
salmonella and meningitides and the last paper was published
in phage therapy. Last year, I made another workshope about
stem cells and its relation with microbiology in 3/3/2015. Last
year I am the adviser of Msc student his work about phage
therapy And now I have another one who work in genotyping
of rotavirus isolated from human and animal in different area
from Iraq.
Detection of diverse virulence factors in phages methicillin
resistance Staphylococcus aureus(MRSA) isolated from mastitic
cows
Atheer Abdulrazzaq Abdulazeez
University of Baghdad, Iraq
Atheer Abdulrazzaq Abdulazeez, Arch Clin Microbiol 2018 Voulume: 9
DOI: 10.4172/1989-8436-C3-011