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Volume 3, Issue 4 (Suppl)
Polym Sci
ISSN: 2471-9935 Polym Sci, an open access journal
October 12-13, 2017 Osaka, Japan
Annual Meeting on
Biopolymers and Drug Delivery Systems
Biopolymers Meeting 2017
October 12-13 2017
Page 59
Polym Sci 2017, 3:4
DOI: 10.4172/2471-9935-C1-006
Nanoformulations for Hsp90 inhibitors for cancer therapy
Ankit K Rochani, Toru Maekawa and D Sakthi Kumar
Toyo University, Japan
H
eat shock protein (Hsp) 90 plays an important role in maintaining protein homeostasis and helps in cancer proliferation. Molecules
targeting Hsp90 in multiple cancers have entered advanced clinical trials. However, Hsp90 targeted cancer therapy faces a major
problem of the aqueous dispensability of drugs due to their hydrophobic nature. Nanoformulation using soft polymers has played an
important role in the aqueous delivery of hydrophobic anticancer drugs. Hence, to address the formulation issue of hydrophobic-Hsp90
targeted drugs, we have developed Hsp90 inhibitors loaded polymeric nanoformulations, which can be dispensed using aqueous phase
(devoid of organic solvents).We have developed 17AAG(first generationHsp90 inhibitor) andFe
3
O
4
loadedPLGAmagnetic nanoparticles
(MNPs). We had developed two types of formulations (1:1:10 and 1:1:20) by relatively varying the concentration of PLGA polymer.
Our study showed that ratio of 1:1:10 for (17AAG, Fe
3
O
4
and PLGA) provided relatively better physicochemical and pharmacological
response. The size of drug loaded polymeric MNPs from 1:1:10 nanoformulation were found to be 204 nm (confirmed with SEM and
TEM images). These NPs provided dual mode of Pancreatic Cancer (MiaPaCa-2) cells destruction under in vitro conditions; rendered
by magnetic hyperthermia (provide by Fe3O4) and Hsp90 inhibition (rendered by 17AAG). We have also developed novel albumin
conjugated Hsp90 inhibitor loaded nanoparticles. We used standard desolvation method for the synthesis of drug conjugated albumin
nanoparticles. Our studies showed that nab-Hsp90 inhibitor nanomedicine was found to have average particle size of around 222 nm
(confirmed with high-resolution SEM and TEM images). Our study shows that the synthesized drug loaded albumin nanoparticles were
found to be effective under in vitro condition against both pancreatic (MiaPaCa-2) and breast cancer (MCF7) cell lines. Our studies
indicate that our nanomedicine platform, which consists of both synthetic and natural (protein based) polymers that can play a significant
role the delivery of Hsp90 targeted anticancer drugs for next-generation anticancer therapy.
anrochani@gmail.com