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Volume 3, Issue 4 (Suppl)
Polym Sci
ISSN: 2471-9935 Polym Sci, an open access journal
October 12-13, 2017 Osaka, Japan
Annual Meeting on
Biopolymers and Drug Delivery Systems
Biopolymers Meeting 2017
October 12-13 2017
Page 58
Polym Sci 2017, 3:4
DOI: 10.4172/2471-9935-C1-006
Optimization of Alpinia galanga oil loaded self-nanoemulsifying drug delivery system using design of
experiments for fish anesthesia
Nattakanwadee Khumpirapang
1
, Anette Müllertz
2
, Thomas Rades
2
and Siriporn Okonogi
1
1
Chiang Mai University, Thailand
2
University of Copenhagen, Denmark
E
thanol used for enhancing water miscibility of the essential oils for fish anesthesia provides undesirable side effects to the fish.
The aim of this study was to develop a water dispersible formulation of Alpinia galanga oil (AGO) self-nanoemulsifying drug
delivery systems (SNEDDS) in order to minimize the amount of ethanol in the formulation and to investigate the effects of the AGO
and AGO-SNEDDS for fish anesthesia. Response surface methodology was used to investigate how excipients affect the droplet size
on AGO-SNEDDS formation. The fish anesthetic activity of AGO-SNEDDS with different droplet sizes was evaluated by the time it
took for zebrafish (Danio rerio) to go into surgical anesthesia stage which fish stopped swimming activity, showed loss of equilibrium
and responsiveness and subsequent recovery. The predicted contour plots of droplet size indicated that cremophor RH 40 provided
smaller droplet size than tween 80. The goodness of model fitting (R2>0.89), prediction power (Q2>0.72) and the droplet size values
between prediction and real measurement showed similar values (% error <10%). Therefore, these models had a good prediction
power. Cremophor RH 40, miglyol 812:capmul MCM EP=1:1 and AGO concentrations showed the most influential variables affecting
the droplet size. The droplet size plays an important role in fish anesthesia. The larger droplet required longer time to take fish to
enter surgical anesthesia stage. SNEDDS3 with a droplet size around 200 nm sedated the fish into the anesthetic stage within 270
sec, significantly slower than SNEDDS1 and SNEDDS2 (218 and 212 sec) with droplet sizes around 60 and 110 nm (p<0.03). All
formulations had significantly increased anesthetic activity compared to AGO in an ethanolic solution. In conclusion, the SNEDDS
are promising nano delivery systems of AGO for anesthetic use in zebrafish.
khumpirapangpearl@gmail.com