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Volume 3, Issue 4 (Suppl)
Polym Sci
ISSN: 2471-9935 Polym Sci, an open access journal
October 12-13, 2017 Osaka, Japan
Annual Meeting on
Biopolymers and Drug Delivery Systems
Biopolymers Meeting 2017
October 12-13 2017
Page 56
Combined use of zoledronic acid augments ursolic acid-induced apoptosis in human osteosarcoma cells
through enhanced oxidative stress and autophagy
Chia-Chieh Wu
1, 2
, Yi-Fu Huang
2
, Yao-Li Chen
2
and Pin-Ju Chueh
1
1
National Chung Hsing University, Taiwan
2
Changhua Christian Hospital, Taiwan
U
rsolic Acid (UA), a naturally occurring pentacyclic triterpene acid found in many medicinal herbs and edible plants, triggers
apoptosis in several tumor cell lines but not in human bone cancer cells. Most recently, we have demonstrated that UA exposure
reduces the viability of human osteosarcoma MG-63 cells through enhanced oxidative stress and apoptosis. Interestingly, an inhibitor
of osteoclast-mediated bone resorption, Zoledronic Acid (ZOL), also a third-generation nitrogen-containing bisphosphonate, is
effective in the treatment of bone metastases in patients with various solid tumors. In this present study, we found that UA combined
with ZOL to significantly suppress cell viability, colony formation, and induce apoptosis in two lines of human osteosarcoma cells. The
pre-treatment of the antioxidant had reversed the oxidative stress and cell viability inhibition in the combined treatment, indicating
that oxidative stress is important in the combined anti-tumor effects. Moreover, we demonstrated that ZOL combined with UA
significantly induced autophagy and co-administration of autophagy inhibitor reduces the growth inhibitory effect of combined
treatment. Collectively, these data shed light on the pathways involved in the combined effects of ZOL and UA that might serve as a
potential therapy against osteosarcoma.
Biography
Chia-Chieh Wu is currently pursuing his PhD degree at the Institute of Biomedical Sciences, National Chung Hsing University in Taiwan.
cedric.jeep@gmail.comChia-Chieh Wu et al., Polym Sci 2017, 3:4
DOI: 10.4172/2471-9935-C1-006