advanced-biotechnology-annual-pediatrics-2018-posters

Page 47

Volume 10

Journal of Archives of Medicine

Advanced Biotechnology & Annual Pediatrics 2018

November 28-29, 2018

Novel Trends and Advances in Biotechnology,

Cell & Stem Cell Research

Annual Congress on Pediatrics

World Congress on

November 28-29, 2018 Barcelona, Spain

Joint Event On

Design of 3D bio-printed scaffolds for cartilage regeneration

G Pinilla, J M Baena, P Gálvez-Martín and J A Marchal

REGEMAT 3D, Spain

artilage is a dense connective tissue with limited self-repair properties. Currently, the therapeutic use of autologous or

allogenic chondrocytes makes up an alternative therapy to the pharmacological treatment. The design of a bio-printed 3D

cartilage with chondrocytes and biodegradable biomaterials offers a new therapeutic alternative able of bridging the limitations

of current therapies in the field. We have developed an enhanced printing processes-Injection Volume Filling (IVF) to increase

the viability and survival of the cells when working with high temperature thermoplastics without the limitation of the scaffold

geometry in contact with cells. We have demonstrated the viability of the printing process using chondrocytes for cartilage

regeneration. This development will accelerate the clinical uptake of the technology and overcomes the current limitation

when using thermoplastics as scaffolds. An alginate-based hydrogel combined with human chondrocytes (isolated from

osteoarthritis patients) was formulated as bioink-A and the polylactic acid as bioink-B. The bioprinting process was carried

out with the REGEMAT V1 bio-printer (Regemat 3D, Granada-Spain) through a IVF. The printing capacity of the bio-printing

plus the viability and cell proliferation of bio-printed chondrociytes was evaluated after five weeks by confocal microscopy

and Alamar Blue Assay (Biorad). Results showed that the IVF process does not decrease the cell viability of the chondrocytes

during the printing process as the cells do not have contact with the thermoplastic at elevated temperatures. The viability and

cellular proliferation of the bio-printed artificial 3D cartilage increased after 5 weeks. In conclusion, this study demonstrates

the potential use of Regemat V1 for 3D bio-printing of cartilage and the viability of bio-printed chondrocytes in the scaffolds

for application in regenerative medicine.

Arch Med 2018, Volume 10

DOI: 10.21767/1989-5216-C2-006