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Page 45
Volume 10 Journal of Archives of Medicine Advanced Biotechnology & Annual Pediatrics 2018
November 28-29, 2018
Novel Trends and Advances in Biotechnology,
Cell & Stem Cell Research
15th Annual Congress on Pediatrics
World Congress on
&
November 28-29, 2018 Barcelona, Spain
Joint Event On
GATA2 directly represses cardiac fates to promote hematopoietic specification of human mesoderm
Giorgetti Alessandra
Center of Regenerative Medicine in Barcelona CMR[B], Spain
In vertebrates, GATA2 is a master regulator of hematopoiesis, repeatedly used throughout embryo development and the
adult life. Although it is well established that GATA2 is essential for the onset of mouse hematopoiesis, its role during
early human hematopoietic development remains elusive. By combining time-controlled overexpression of GATA2 with
genetic knockout experiments, we found that GATA2, at the mesoderm specification stage, promotes the generation of hemo-
genic progenitors and their further differentiation to hematopoietic progenitor cells, while negatively regulating cardiac
differentiation. Surprisingly, genome-wide transcriptional and chromatin immune-precipitation analysis showed that GATA2
bound preferentially to regulatory regions, and repressed expression, of cardiac development-related genes. In contrast, genes
important for hematopoietic differentiation were up-regulated by GATA2 in a mostly indirect manner. Collectively, our
data reveal a previously unsuspected role of GATA2 as a direct repressor of cardiac fates, and highlight the importance of
coordinating the specification and repression of alternative cell fates.
Arch Med 2018, Volume 10
DOI: 10.21767/1989-5216-C2-006