Phytochemical Screening and Evaluation of In vitro Antioxidant Activity of Viburnum punctatum buch-ham. Ex d.don

The present study was to estimate the preliminary phytochemical screening and in vitro anti-oxidant activity of aerial part extracts of Viburnum punctatum by using solvents like Petroleum ether, Chloroform, Methanol and Water. Preliminary phytochemical analysis reveals the presence of alkaloids, glycosides, flavonoids, phenolic compounds, proteins, phytosterols and saponins. The chloroform and methanol extract were screened for its potential antioxidant activity by DPPH radical scavenging activity, Hydroxyl radical scavenging activity, Nitric oxide scavenging activity, Reducing power activity and Superoxide free radical scavenging activity using Ascorbic acid as a standard. The results of these five methods anti-oxidant study indicate ta concentration dependent antioxidant activity of various concentrations of 50μg/ml, 100μg/ml and 200μg/ml of the extract respectively. The DPPH radical scavenging activity and other radical scavenging activities of the extract was increased with increasing concentration. The methanol extract exhibited better anti-oxidant activity than chloroform extract for all the five in vitro anti-oxidant activity screened.

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