Human Umbilical Cord Tissue Derived Mesenchymal Stem Cells and Stem Cell Conditioned Medium and their potentials towards regenerative application

Stem-cell therapy specially the umbilical cord-mesenchymal stem cells (UC-MSCs) is a emerging alternative to treat progressing tissue damage by reorganising the underlying disease process and to repair injured tissues, home to inflamed sites and involves least ethical concerns. Numerous questions need to be justified before MSCs derived from UC and their conditioned-medium can be applicable for the therapy of diverse diseases/conditions. Stromal cells from human umbilical cord were characterised for CD29, CD105, CD73, CD166, CD271 and CD44 expression, CFU-F potential, wound assay potential and tri-lineage differentiation potential. Gene expression of Sox9, Oct4, Nanog, Colla1a1, Fmod, Nestin, Angplt1, CXCL2, IGF1, CDK6, p21, CyclinD1 and Wnts, were analysed in UCMSCs. UCMSC conditioned-medium was obtained after 7 days culture of UCMSCs and was characterized by HPLC, GC-MS and cytokine immune assay and thereafter treated in human keratinocyte cells (HaCaT) and co cultured with colon cancer cells for 10-12 days.

Author(s): Antara Banerjee

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