E Lesniewska, M J Virolle, E Aybeke, A Deniset-Besseau, N Pocholle, E Bourillot and A Dazzi
ICB UMR CNRS 6303, University of Bourgogne, France I2BC UMR 9198, Universite Paris-Sud, France Universite Paris-Sud, France
ScientificTracks Abstracts: Nano Res Appl
We have developed a new AFM-based platform combining IR spectroscopy and acoustic and/or microwave 3D tomography to detect and characterize lipidic vesicles present in the cytoplasm of various micro-organisms. We started with the bacteria Streptomyces that is able to store excess of carbon as triacylglycerols (TAGs) in lipid vesicles. TAG are a ready-to-use source of biodiesel, chemically and structurally identical to those found in commercial fuels. To illustrate the potential of these techniques, we will present the detection and size distribution (accuracy under 10 nm) of triglycerides vesicles in Streptomyces using high-resolution infrared microscopy AFM-IR as well as acoustic wave in ultrasound mode UA-AFM. We extended the excitation range to microwave (range up to 16 GHz) and achieved a comparative study of AFM-IR, acoustic and microwave scanning analysis. Our results indicate that the coupling of these techniques constitutes a great advantage to fully characterize chemical, topographical and volumetric parameters of a biological sample. We will present a 3D reconstruction of bacteria or yeast cells, showing the in-depth vesicles distribution. Similar analysis will be carried out with oleaginous (Yarrovia lipolytica) and non-oleaginous yeasts (Saccharomyces cerevisiae yeasts) as well as with Listeria in order to demonstrate the great potential of acoustic and microwave microscopy.
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