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Volume 3, Issue 2 (Suppl)
Trends in Green chem
ISSN: 2471-9889
Environmental & Green Chemistry 2017
July 24-26, 2017
Page 97
5
th
International Conference on
6
th
International Conference on
July 24-26, 2017 Rome, Italy
Environmental Chemistry and Engineering
Green Chemistry and Technology
&
Accelerated solvent extraction as an alternative tool for extraction of chlorogenic acid from Cynara
scolymus leaves
Ibrahim Ahmed Saleh
1
, Nahla Sayed Abdel-Azim
1
, Khaled Ahmed Shams
1
, Elsayed Aboutabl
2
, Faiza Mohamed Hammouda
1
, Mohamed-Elamir Fathy Hegazy
1
and
Tarik Abdelhalim Mohamed
1
1
National Research Centre (NRC), Egypt
2
Cairo University, Egypt
F
inding new techniques to enhance the extraction efficiency of bioactive compounds frommedicinal and aromatic plants (MAPs) is
important due to their potential health benefits. The objective of this study was to evaluate extractability of chlorogenic acid form
Cynara scolymus leaves using solvents under high pressure and temperature. Accelerated Solvent Extraction (ASE) is considered as
a greener method for MAPs extraction when compared to conventional extraction technique. ASE is an extraction technique which
combines both elevated pressure and temperature. Methodology &Theoretical Orientation: Chlorogenic acid form Cynara scolymus
leaves was extracted using ASE applying one and three static cycles of extraction at 40, 80, and 120 °C. Static periods of extraction were
carried out for 5, 10 and 15 min using 80% methanol/water (v/v) as the extraction solvent. The extracted solutions were characterized
for chlorogenic acid content by HPLC. Findings: The highest chlorogenic acid yields were obtained after 10 min (67.4 and 66.8 mg/5g
DM) using one and three static cycles of extraction; respectively. Accelerated Solvent Extraction (ASE) has proven to be an effective
extraction technique. However its recommended not to exceed the extraction time over 10 min at elevated temperatures, due to the
possible degradation of chlorogenic acid for prolonged extraction at extreme conditions.
Biography
Ibrahim Saleh, Researcher, Phytochemistry Department, National Research Centre, Egypt, has awarded his Ph.D degree from faculty of pharmacy, Cairo University,
Egypt. He has awarded international scholarship as an academic visitor, Coventry University, Coventry, United Kingdom. During the last ten years, he has been
publishing in the area of natural products chemistry, his program has come to focus on innovation and development of medicinal and aromatic plant processing.
brahim82@hotmail.comIbrahim Ahmed Saleh et al., Trends in Green chem, 3:2
DOI: 10.21767/2471-9889-C1-003