environmental-green-chemistry-2017-posters-abstracts

Notes:

Volume 3, Issue 2 (Suppl)

Trends in Green chem

ISSN: 2471-9889

Environmental & Green Chemistry 2017

July 24-26, 2017

Page 95

International Conference on

July 24-26, 2017 Rome, Italy

Environmental Chemistry and Engineering

Green Chemistry and Technology

Enhanced GABA production via protein complex of

Pyrococcus horikoshii

glutamate decarboxylase and

Escherichia coli GABA transporter

Soon Ho Hong

University of Ulsan, Korea

amma-aminobutyric acid (GABA) is a precursor to pyrrolidone, a monomer used for the production of a biodegradable polymer

known as nylon-4. GABA is also widely used in the medical industry to treat conditions such as high blood pressure, anxiety and

depression. Generally, GABA is produced from glutamate by the enzyme glutamate decarboxylase (GadB). In this study, a synthetic

scaffold complex was introduced between

Pyrococcus horikoshii

GadB and the GABA antiporter (GadC) from

E. coli. P. horikoshii

GadB was attached to the N-terminus, C-terminus and middle of

E. coli

GadC via scaffolding. Among the three scaffold complexes

evaluated, the N-terminus scaffold model produced 5.93 g/L of GABA from 10 g/L monosodium glutamate (MSG). When the gabT

mutant

E. coli

XBT strain was used, the highest GABA concentration of 5.96 g/L was obtained, which is 97.8% of GABA yield. In

addition to GABA concentration, GABA productivity was increased 3.5 fold via the synthetic scaffold complex.

Biography

Soong Ho Hong graduated from School of Chemical Engineering & Bioengineering, University of Ulsan, Korea. His major research field involves Metabolic

Engineering, In Silico Simulation of Microorganisms, Systems Biology, Synthetic Biology, and Bioengineering.

Soon Ho Hong, Trends in Green chem, 3:2

DOI: 10.21767/2471-9889-C1-003