The present study was performed to evaluate the anti-ulcer activity of methanolic extract of Hyoscyamus albus and Umbilicus rupestris against ethanol-induced gastric ulcer. Experimental groups were orally pre-treated with and 100, and 200 mg/kg b.w of methanolic extracts HAMeOH and URMeOH. Ulcer control group were pre-treated just with absolute ethanol and reference group was orally pretreated with omeprazole (20 mg/kg b.w). After one hour of pretreatment, all rats were received the absolute ethanol to generate gastric mucosal injury. And after one hour, our rats were anesthetized and sacrificed and the ulcer areas of the gastric walls were determined. Grossing evaluation has revealed that the negative control rats exhibited severe mucosal injury, whereas, pre-treatment with U. rupestris leaf extract and H. albus has resulted significantly anti gastric mucosal injury and flattening of the mucosal folds. Histological studies of the gastric wall that the pre-treated with URMeOH and HAMeOH leaf extract was pronounced as gastric protection along followed by reduction or inhibition of leucocytes in the infiltration of the submucosa.