The use of bioactive compounds in different commercial sectors such as pharmaceutical, food and chemical industries assures the need of the most appropriate and standard method to extract these active components from plant materials. In the present study, conventional methods and numerous new methods (maceration, reflux, soxhlet, hydrodistillation, Ultrasound-Assisted Extraction (UAE) and Microwave-Assisted Extraction (MAE)) using different solvents have been developed for the extraction of bioactive compounds from Urtica dioica grown in Lebanon. Our results revealed that the extraction method, solvent and time had a significant effect on the amount of the extracted compounds. In terms of extraction method applied, microwave-assisted extraction was the more effective technique compared to the other methods. The extraction time was reduced, less solvent was used and the amount of extracted compounds was increased.
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