This work as performed to investigates seed proximate composition, and oil content, oil physical, chemical properties, and constituents of Eruca sativa. The results of Eruca sativa seed chemical composition are; Oil content (20%), moisture content (3.64c), Crude protein (31.0), Crude fibers (20.376%), Total ash (4.33%) and Total carbohydrates (23.07). Physiochemicals of Eruca sativa oil are; relative density (90,77%), refractive index (1.469), relative viscosity (38cp), peroxide value (10), Acid value (2.24), Colour (red 5.4–yellwo 3.1–blue 0.4), saponnification value (165.495), unsaponnification value (2.648%), iodine value (63.63). GC-MS analysis showed 28 oil constituents in Eruca sativa oil. Palmatic acid (hexadecanoic acid), Stearic acid (octadecanoic acid), Oleic acid (Octadecenoic acid), Linoleic acid as (Octadecadienoic acid), Erucic acid (Docosenoic acid).