Silver nanoparticles were synthesized by a bacterial isolate, retrieved from soil which was identified as Agrococcus sp. The bacterial culture was isolate by enrichment culture technique using AgNO3 in LB medium. Silver nanoparticles were characterized by using transmission electron microscopy. It was observed that bacterial isolate produced silver nanoparticles in size ranged of 5-80 nm. Particle size analyzers gave the average diameter of silver nanoparticles as 555.1. Further, by using UV-Visible spectrophotometer culture broth showed silver surface Plasmon band at 311nm. FTIR (Fourier transform infrared spectroscopy) analysis showed four major peaks at 3450.47 cm-1, 2077.67 cm-1, 1636.91 cm- 1 and 1017.70 cm-1.