Reach Us +44 1704 335730
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

IL-10-Producing Th17 Cells:A Potentially Regulatory Cell Population in Dry Eye Disease

Purpose: To assess the effect of dry eye disease (DED) on regulatory IL-10- producing Th17 cell population.

Methods: DED was induced in female C57BL/6 mice. mRNA and protein expression levels of IL-17 and IL-23 were analyzed in the conjunctivae and draining lymph nodes (DLNs) of mice 14 and 28 days after DED induction using real-time PCR and ELISA, respectively. The frequencies of IL-17-producing Th17 (IL-17+CD4+) cells and IL-10-producing Th17 (IL-10+IL-17+CD4+) cells were determined in the conjunctivae and DLNs of DED mice on days 14 and 28 using flow cytometry analysis.

Results: DED induction significantly upregulated the expression of IL-17 and IL-23 at the ocular surface and draining lymph nodes at day 14. Frequencies of IL-17 producing Th17 cells significantly increased in mice with acute DED, and returned to near normal levels with disease resolution. These changes were accompanied by a significant decrease in frequencies of IL-10-producing Th17 cells in acute DED, and their restoration to baseline levels with resolution of DED.

Conclusions: Decreased frequencies of IL-10-producing Th17 cells concomitant with increased effector Th17 cell frequencies and IL-17 and IL-23 cytokine levels contribute to DED pathogenesis.


Author(s): Hong Qi, Yihe Chen, Takenori Inomata, Afsaneh Amouzegar and Reza Dana

Abstract | Full-Text | PDF

Share this  Facebook  Twitter  LinkedIn  Google+