Hardwickia binata Roxb. (Fabaceae) root bark exudate has been traditionally used by tribes of Chitteri hills to cure breast cancer. The main objective of this study is to give a scientific validation to the traditional use of H. binata. The root bark exudates of H. binata was collected from Chitteri hills, shade dried, coarsely powdered and extracted with methanol using soxhlet apparatus. The Physico chemical properties, qualitative phytochemical studies, quantitative phytochemical studies and GC-MS analysis was carried out. The cytotoxic effect of methanol extract of H.binata root bark exudate was studied against cancer cell lines by MTT assay. The phytochemical studies indicated the presence of flavonoids, saponins, phenols and tannins. GC-MS study also revealed the presence of 22 organic compounds out of which 18 compounds were reported with various activities and five compounds reported to possess anticancer activity. In vitro cytotoxic activity on the cell lines showed more degree of inhibition against African Green Monkey Kidney Epithelial cells (Vero), Human cervical cancer cell lines (HeLa) and Human breast cancer cells (MCF-7).
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