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Structural Biology 2018
Volume: 4
Biochemistry & Molecular Biology Journal
Page 44
March 15-16 2018
Barcelona, Spain
10
th
Edition of International Conference on
Structural Biology
N
uclear magnetic resonance (NMR) is a powerful technique
which enables characterization of various biomolecules
in solution. This way one is able to track more accurately the
dynamic properties and changes in the structure over time and
varying conditions (temperature, salt concentration, ligand
addition, and pH). Psb proteins of photosystem II are a class of
extrinsic proteins located on the cytosolic part of the oxygen
evolving center. They help to maintain proper conditions for
the water splitting reaction ultimately leading to the release of
molecular oxygen. We have studied so far three representatives:
PsbP (23 kDa), PsbQ (16 kDa) and PsbO (33 kDa). Using NMR
we have been able to determine 3D structures of PsbP and
PsbQ in solution including their highly flexible regions whose
characterization was hindered with the use of other techniques
(X-ray and Cryo-electron microscopy). The most interesting
information about detailed interplay of those proteins and its
dependence on the presence of metal cations has been studied
using combination of techniques, such as titration and chemical
exchange NMR technique (CEST), isothermal titration calorimetry
(ITC) and microscale thermophoresis (MST).
Biography
Adriana Rathner obtained her Double Bachelor’s Degree in Biological Chem-
istry at Johannes Kepler University Linz (Austria) and the University of South
Bohemia (Czech Republic) in 2010. Her Bachelor’s thesis dealt with the opti-
mization of synthesis of dipeptides for isotope labeling and pilot expression
of outer surface protein from pathogenic Borrelia, respectively. She then
continued in the Joint Master’s Program of Biological Chemistry and within
her thesis, worked with recombinant photosynthetic protein PsbP and its
initial NMR characterization in the group of Professor Norbert Müller (JKU
Linz). Since 2013, she is a PhD student in the same group with the main
focus on large scale production of isotopically labeled proteins of photo-
system II and their subsequent study by various solution nuclear magnetic
resonance (NMR) techniques.
adriana.rathner@jku.atStudy of extrinsic Psb proteins of photosystem II by solution
nuclear magnetic resonance
Adriana Rathner
1
, Michal Kamenický
1
, Michaela Horničáková
1
, Kousik Chandra
1
, Norbert
Müller
1
, Jaroslava Kohoutová
2
, Rüdiger Ettrich
2
, Linda Cerofolini
3
and
Claudio Luchinat
3
1
Johannes Kepler Universität Linz, Austria
2
Academy of Sciences of the Czech Republic, Czech Republic
3
University of Florence, Italy
Adriana Rathner et al., Biochem Mol biol J, Volume 4
DOI: 10.21767/2471-8084-C1-009