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Polymer Congress 2018 Polymer Sciences
ISSN: 2471-9935 Page 79
June 04-05, 2018
London, UK
4th Edition of International Conference on
Polymer Science and
Technology
Nitric oxide (NO) is a small molecule involved in a wide
range of physiological and pathophysiological processes,
including vasodilatation, control of inflammatory pain, wound
healing, and antibacterial activities. As NO is a free radical,
the design of drugs that generates therapeutic amounts of
NO in controlled spatial and time manners is still a challenge.
In this study, the NO donor S-nitrosoglutathione (GSNO) was
incorporated into the thermoresponsive pluronic F-127 (PL) -
chitosan (CS) hydrogel, in an easy and economically feasible
methodology. CS is a polysaccharide with known antimicrobial
and biocompatibility properties. Scanning electron microscopy,
rheology and differential scanning calorimetry techniques were
used for hydrogel characterization. The results demonstrated
that the hydrogel has a smooth surface, thermoresponsive
behavior, and good mechanical stability. The kinetics of
NO release and GSNO diffusion from GSNO-containing PL/
CS hydrogel demonstrated a sustained NO/GSNO release,
in concentrations suitable for biomedical applications, at
physiological and skin temperatures. The GSNO-PL/CS
hydrogel demonstrated a concentration-dependent toxicity
to Vero cells and antimicrobial activity to Pseudomonas
aeruginosa (minimum inhibitory concentration and minimum
bactericidal concentration values of 0.5 µg•mL-1 of hydrogel,
which correspondents to 1 mmol•L-1 of GSNO). Interestingly,
the concentration range in which the NO-releasing hydrogel
demonstrated antibacterial effect was not found toxic to
Vero mammalian cell. Thus, GSNO-PL/CS hydrogel is suitable
biomaterial for topical NO delivery applications.
Pluronic F-127 and chitosan hydrogel delivering nitric oxide
with antibacterial effect and cytocompatibility
Milena T Pelegrino1, Bruna de Araujo Lima2, Mônica H M do Nascimento1, Chris-
tiane B Lombello1 , Marcelo Brocchi2 and Amedea B Seabra1
1UFABC, Brazil
2UNICAMP, Brazil
Polym Sci 2018, Volume 4
DOI: 10.4172/2471-9935-C2-012