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Volume 3, Issue 4 (Suppl)
J Clin Exp Orthop
ISSN:2471-8416
Osteoporosis and Arthroplasty 2017
December 04-05, 2017
&
11
th
International Conference on
Joint Event
OSTEOPOROSIS, ARTHRITIS & MUSCULOSKELETAL DISORDERS
December 04-05, 2017 | Madrid, Spain
10
th
INTERNATIONAL CONFERENCE ON ARTHROPLASTY
Ankle cartilage is more resilient to cytokine-induced catabolism than knee cartilage: Apotential target for
prevention of knee arthritis?
Ali Abdullah
Cardiff University, UK
Introduction &Aim:
The variation in prevalence of osteoarthritis has been hypothesized to result from the differential responsiveness
of joints to catabolic stimuli; therefore the aim of this study was to determine whether ankle cartilage is less susceptible to the catabolic
effects of pro-inflammatory cytokines when compared to the knee.
Methods:
Human cartilage explants were taken from the talar domes (n=12) and the femoral condyles (n=7) following surgical
amputation. Explants were cultured in the presence or absence of either a combination of high or low concentration of cytokines, and
media analyzed up to 28 days. Sulphated glycosaminoglycan (sGAG) release to the media and expression levels of nitric oxide and
prostaglandin E
2
(PGE
2
) were measured.
Results:
Significantly more sGAG was lost from knee cartilage explants exposed to 100 ng/ml TNFα (22.2% vs. 13.2%, P=0.01)
and 100 ng/ml TNFα in combination with 5 ng/ml IL-1α (27.5% vs. 16.0%, P=0.02) compared to sGAG release from the ankle; low
cytokine concentrations did not affect sGAG release. All high concentration cytokine treatments resulted in production of more
nitrite and PGE
2
compared to low concentrations; however, no significant differences between the knee and ankle were noted for
nitrite although there was significantly more PGE
2
production in knee cartilage.
Discussion:
Cartilage explants from the knee and ankle have a divergent response to stimulation by pro-inflammatory cytokines,
with high concentrations of TNFα alone, or in combination with IL-1α amplifying cartilage degeneration. This differential response
may account for the high prevalence of knee arthritis compared to ankle OA and provide a future pharmacological target to treat OA
of the knee.
Biography
Ali Abdullah is a 5th year Medical student and has attained a BSc in Biomedical Sciences (Anatomy) from Cardiff University. He has previously presented research
at various national conferences.
a.a.n.abdullah123@gmail.comAli Abdullah, J Clin Exp Orthop 2017, 3:4(Suppl)
DOI: 10.4172/2471-8416-C1-002