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Infectious Diseases
and STD-AIDS
Infectious Diseases and STD-AIDS 2018
Journal of Transmitted Diseases and Immunity
ISSN 2471-8084
A p r i l 2 6 , 2 7 2 0 1 8
R o m e , I t a l y
Page 48
J Transm Dis Immun 2018 Volume 2
DOI: 10.21767/2573-0320-C1-003
Background:
Tuberculosis (TB) is still a serious global public health problemand a leading cause ofmorbidity andmortality through-
out the world. Amajor factor driving the spread of tuberculosis is the considerable delay in diagnosis and treatment. For this reason,
early detection is paramount. The present study was carried out to compare the automated BACTEC MGIT 320 with conventional
LJ culture media and direct AFB smear examination.
Methods:
This prospective study was carried out in Amhara Public Health Institute (APHI) microbiology department tuberculosis
laboratory which is equipped with gene expert, BACTEC™MGIT™ 320 and Löwenstein-Jensen (LJ) culture infrastructures over a pe-
riod of nine months, fromNovember 2016 to July 2017. A total of 282 consecutive clinical sputum samples were obtained through
postal system from 273 follow up and nine new suspected cases of TB from different parts of Amhara region.
Results:
A total of 282 sputum samples were processed on conventional LJ solid media, MGIT 320 and direct AFB smear exam-
ination methods. Out of 282 specimens, 50 (17.7%) were positive with either MGIT or conventional culture from which 16 (32%)
were smear positive and 34 (68%) were smear negative. The recovery rate of MGIT and LJ from smear positive samples was 77.8%
(14/18) and 66.7% (12/18), respectively while smear negative samples was 11.7% (31/264) and 4.5% (12/264) for MGIT and LJ
methods, respectively. On comparing the overall mycobacterial recovery rate of MGIT and LJ culture, 45 (16%) were positive with
MGIT-320 whereas 24 (8.5 %) were positive with LJ culture. TheMDGIT320 has improved significantly the recovery rate of M. tuber-
culosis complex (MTBC) (P<0.005). For MGIT, 88% Mycobacterium were recovered within the first two weeks whereas for LJ, 76%
were recovered within fourth to eight weeks. Relatively fair agreement has been observed between MGIT 320 and LJ methods with
Kappa value of 0.277. Contamination rates for MGIT and LJ were 18.1% and 4.6% respectively.
Conclusion:
The BACTECMGIT320 liquid culture systemshowed better performance than conventional LJmethod for rapid recov-
ery of Mycobacterium tuberculosis complex with shorter turnaround time for both smear positive and negative clinical specimens.
However, the high contamination rate is a concern that should be carefully managed.
deresehailu86@gmail.com
Evaluation of the BACTEC MGIT 320 TB system for
the recovery and identification of Mycobacterium
tuberculosis at Amhara public health institute,
Bahir Dar, north west Ethiopia
Derese Hailu, Gashaw Yitayew, Yosef Gashaw, Eyaya Wondi-
fraw, Kefale Ejigu and Gizachew Yismaw
Amhara Public Health Institute, Ethiopia