11 / 38
Infectious Diseases
and STD-AIDS
Infectious Diseases and STD-AIDS 2018
Journal of Transmitted Diseases and Immunity
ISSN 2471-8084
A p r i l 2 6 , 2 7 2 0 1 8
R o m e , I t a l y
Page 47
J Transm Dis Immun 2018 Volume 2
DOI: 10.21767/2573-0320-C1-003
Background:
Tuberculosis (TB) is still a serious global public health problem and a leading cause of morbidity and mortality
throughout the world. A major factor driving the spread of tuberculosis is the considerable delay in diagnosis and treatment. For
this reason, early detection is paramount. The present study was carried out to compare the automated BACTEC MGIT 320 with
conventional LJ culture media and direct AFB smear examination.
Methods:
This prospective study was carried out in Amhara Public Health Institute (APHI) microbiology department tuberculosis
laboratory which is equipped with gene expert, BACTEC™ MGIT™ 320 and Löwenstein-Jensen (LJ) culture infrastructures over
a period of nine months, from November 2016 to July 2017. A total of 282 consecutive clinical sputum samples were obtained
through postal system from 273 follow up and nine new suspected cases of TB from different parts of Amhara region.
Results:
A total of 282 sputum samples were processed on conventional LJ solid media, MGIT 320 and direct AFB smear exam-
ination methods. Out of 282 specimens, 50 (17.7%) were positive with either MGIT or conventional culture from which 16 (32%)
were smear positive and 34 (68%) were smear negative. The recovery rate of MGIT and LJ from smear positive samples was
77.8% (14/18) and 66.7% (12/18), respectively while smear negative samples was 11.7% (31/264) and 4.5% (12/264) for MGIT
and LJ methods, respectively. On comparing the overall mycobacterial recovery rate of MGIT and LJ culture, 45 (16%) were posi-
tive with MGIT-320 whereas 24 (8.5 %) were positive with LJ culture. The MDGIT320 has improved significantly the recovery rate
of
M. tuberculosis
complex (MTBC) (P<0.005). For MGIT, 88%
Mycobacterium
were recovered within the first two weeks whereas
for LJ, 76% were recovered within fourth to eight weeks. Relatively fair agreement has been observed between MGIT 320 and LJ
methods with Kappa value of 0.277. Contamination rates for MGIT and LJ were 18.1% and 4.6% respectively.
Evaluation of the BACTEC MGIT 320 TB system for
the recovery and identification of Mycobacterium
tuberculosis at Amhara public health institute,
Bahir Dar, north west Ethiopia
Derese Hailu, Gashaw Yitayew, Yosef Gashaw, Eyaya
Wondifraw, Kefale Ejigu and Gizachew Yismaw
Amhara Public Health Institute, Ethiopia
Conclusion:
The BACTEC MGIT 320 liquid culture system showed better performance than conventional LJ method for rapid
recovery of
Mycobacterium tuberculosis
complex with shorter turnaround time for both smear positive and negative clinical
specimens. However, the high contamination rate is a concern that should be carefully managed.
deresehailu86@gmail.com